With all these problems around antibodies, how do you choose the appropriate method?
First, you need to decide which test you will use antibodies. For example, the CRISPR-CAS9 method does not involve the modification of target proteins and validate that antibodies do not have cross-reactivity with other proteins.
So, you can use this technique to validate antibodies for a variety of testing, including Western Blot, IHC, Immunocytochem (ICC), Flow Cytometry, Elisa, Immunoprecipitation (IP), Chromatin Immunoprecipitation (Chip), and a reverse-phase protein test. There are many companies like bosterbio that can help you out with appropriate antibody validation methods.
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Second, researchers must be aware of the limitations of the sample of the validation method. For example, either the technique of protein expression of crispr-cas9 / ko and tagged cannot be used to validate antibodies in human tissue samples and body fluids, such as plasma and serum.2 that is because you cannot manipulate genetic in humans, unlike in cell lines,
Finally, regardless of the antibody method and each validation used by an antibody provider, researchers must do at least one validation strategy in their special application or sample context.
If you are in doubt and want to get more details about antibody validation then you may leave a comment and we will answer you shortly.